The target analytes in liquid chromatography often elute in a very small time slot. The matrix-related substances that elute later are of no interest to the analyst, but put a strain on the mass spectrometer. Normally this has to be awaited, as well as the reconditioning of the column.
With the CHRONECT Workstation Multiplexing a solution for better utilization of LC-MS systems was developed. It allows up to four LC columns and pumps to be installed in parallel. By intelligent valve switching, only the target analytes are transferred to the mass spectrometer. The rest of the matrix does not load the mass spectrometer. There is also no loss of time for reconditioning, since the next measurement can already be started with another column. By using up to four HPLC channels in parallel, the desired sections of four samples can be transferred to the LC-MS in a time-interleaved manner and analyzed in close succession. Thus, the sample throughput of LC-MS systems can be increased up to fourfold.
The basis is a CHRONECT Robotic Autosampler and the HPLC pump SPH1299 from Spark. The entire control and intelligent time management is handled by the CHRONOS software platform.